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a multiband emission filter designed for gfp and mcherry  (IDEX)

 
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    IDEX a multiband emission filter designed for gfp and mcherry
    ( a ) A simplified schematics illustrating LBS generation. L1: Collimating lens; CL1 and CL2: a pair of cylindrical lenses shaping the beam appropriately to maximize the energy passing through the single slit; L2: Fourier transform lens; EO: Excitation objective; DO: Detection objective. The beam is defined as propagating along y-axis. Using custom designed annuli one can craft LBS optimized for ( b ) ultralow phototoxicity (LBS1) or ( c ) high resolution (LBS2). In ( b , c ) from left to right: the annulus used and its numerical apertures (NA): NA max and NA min ; the diffraction pattern after the annulus; the cross-section of the LBS generated at sample plane; the intensity plot (blue) along the dotted yellow line and the axial point spread function of the NA = 1.1 detection objective (red); and the axial plot of overall point spread function. ( d , e ) show the experimentally generated LBSs at different wavelengths (488 nm <t>for</t> <t>GFP</t> and 560 nm for <t>mCherry).</t> The point spread functions were measured with 20 nm fluorescent beads [left two columns in ( d , e )], which are in good agreement with the theoretical plot in ( b , c ). ( f ) The measured FOV profile of Gaussian light-sheet, LBS1 and LBS2. The Gaussian light-sheet is generated by taking away the annulus and confining the maximum NA to 0.5 so that the beam waist has a thickness of 600 nm, which is comparable to the thickness of LBS1. Scale bars in ( d , e ): 5 μm.
    A Multiband Emission Filter Designed For Gfp And Mcherry, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+multiband+emission+filter+designed+for+gfp+and+mcherry/a+multiband+emission+filter+designed+for+gfp+and+mcherry/pmc04870613-13-8-9
    Average 90 stars, based on 1 article reviews
    a multiband emission filter designed for gfp and mcherry - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets"

    Article Title: Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets

    Journal: Scientific Reports

    doi: 10.1038/srep26159

    ( a ) A simplified schematics illustrating LBS generation. L1: Collimating lens; CL1 and CL2: a pair of cylindrical lenses shaping the beam appropriately to maximize the energy passing through the single slit; L2: Fourier transform lens; EO: Excitation objective; DO: Detection objective. The beam is defined as propagating along y-axis. Using custom designed annuli one can craft LBS optimized for ( b ) ultralow phototoxicity (LBS1) or ( c ) high resolution (LBS2). In ( b , c ) from left to right: the annulus used and its numerical apertures (NA): NA max and NA min ; the diffraction pattern after the annulus; the cross-section of the LBS generated at sample plane; the intensity plot (blue) along the dotted yellow line and the axial point spread function of the NA = 1.1 detection objective (red); and the axial plot of overall point spread function. ( d , e ) show the experimentally generated LBSs at different wavelengths (488 nm for GFP and 560 nm for mCherry). The point spread functions were measured with 20 nm fluorescent beads [left two columns in ( d , e )], which are in good agreement with the theoretical plot in ( b , c ). ( f ) The measured FOV profile of Gaussian light-sheet, LBS1 and LBS2. The Gaussian light-sheet is generated by taking away the annulus and confining the maximum NA to 0.5 so that the beam waist has a thickness of 600 nm, which is comparable to the thickness of LBS1. Scale bars in ( d , e ): 5 μm.
    Figure Legend Snippet: ( a ) A simplified schematics illustrating LBS generation. L1: Collimating lens; CL1 and CL2: a pair of cylindrical lenses shaping the beam appropriately to maximize the energy passing through the single slit; L2: Fourier transform lens; EO: Excitation objective; DO: Detection objective. The beam is defined as propagating along y-axis. Using custom designed annuli one can craft LBS optimized for ( b ) ultralow phototoxicity (LBS1) or ( c ) high resolution (LBS2). In ( b , c ) from left to right: the annulus used and its numerical apertures (NA): NA max and NA min ; the diffraction pattern after the annulus; the cross-section of the LBS generated at sample plane; the intensity plot (blue) along the dotted yellow line and the axial point spread function of the NA = 1.1 detection objective (red); and the axial plot of overall point spread function. ( d , e ) show the experimentally generated LBSs at different wavelengths (488 nm for GFP and 560 nm for mCherry). The point spread functions were measured with 20 nm fluorescent beads [left two columns in ( d , e )], which are in good agreement with the theoretical plot in ( b , c ). ( f ) The measured FOV profile of Gaussian light-sheet, LBS1 and LBS2. The Gaussian light-sheet is generated by taking away the annulus and confining the maximum NA to 0.5 so that the beam waist has a thickness of 600 nm, which is comparable to the thickness of LBS1. Scale bars in ( d , e ): 5 μm.

    Techniques Used: CRAfT Assay, Generated

    Related Articles

    Blocking Assay:

    Article Title: Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets
    Article Snippet: The fluorescent signal from the sample is collected by the detection objective and focused on to sCMOS camera (Hamamatsu) by a tube lens with f = 600 mm. .. A multiband emission filter designed for GFP and mCherry (Semrock) is placed in front of the tube lens to block unwanted illumination from the excitation laser. ..



    Similar Products

    a multiband emission filter designed for gfp and mcherry  (IDEX)
    90
    IDEX a multiband emission filter designed for gfp and mcherry
    ( a ) A simplified schematics illustrating LBS generation. L1: Collimating lens; CL1 and CL2: a pair of cylindrical lenses shaping the beam appropriately to maximize the energy passing through the single slit; L2: Fourier transform lens; EO: Excitation objective; DO: Detection objective. The beam is defined as propagating along y-axis. Using custom designed annuli one can craft LBS optimized for ( b ) ultralow phototoxicity (LBS1) or ( c ) high resolution (LBS2). In ( b , c ) from left to right: the annulus used and its numerical apertures (NA): NA max and NA min ; the diffraction pattern after the annulus; the cross-section of the LBS generated at sample plane; the intensity plot (blue) along the dotted yellow line and the axial point spread function of the NA = 1.1 detection objective (red); and the axial plot of overall point spread function. ( d , e ) show the experimentally generated LBSs at different wavelengths (488 nm <t>for</t> <t>GFP</t> and 560 nm for <t>mCherry).</t> The point spread functions were measured with 20 nm fluorescent beads [left two columns in ( d , e )], which are in good agreement with the theoretical plot in ( b , c ). ( f ) The measured FOV profile of Gaussian light-sheet, LBS1 and LBS2. The Gaussian light-sheet is generated by taking away the annulus and confining the maximum NA to 0.5 so that the beam waist has a thickness of 600 nm, which is comparable to the thickness of LBS1. Scale bars in ( d , e ): 5 μm.
    A Multiband Emission Filter Designed For Gfp And Mcherry, supplied by IDEX, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+multiband+emission+filter+designed+for+gfp+and+mcherry/a+multiband+emission+filter+designed+for+gfp+and+mcherry/pmc04870613-13-8-9
    Average 90 stars, based on 1 article reviews
    a multiband emission filter designed for gfp and mcherry - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) A simplified schematics illustrating LBS generation. L1: Collimating lens; CL1 and CL2: a pair of cylindrical lenses shaping the beam appropriately to maximize the energy passing through the single slit; L2: Fourier transform lens; EO: Excitation objective; DO: Detection objective. The beam is defined as propagating along y-axis. Using custom designed annuli one can craft LBS optimized for ( b ) ultralow phototoxicity (LBS1) or ( c ) high resolution (LBS2). In ( b , c ) from left to right: the annulus used and its numerical apertures (NA): NA max and NA min ; the diffraction pattern after the annulus; the cross-section of the LBS generated at sample plane; the intensity plot (blue) along the dotted yellow line and the axial point spread function of the NA = 1.1 detection objective (red); and the axial plot of overall point spread function. ( d , e ) show the experimentally generated LBSs at different wavelengths (488 nm for GFP and 560 nm for mCherry). The point spread functions were measured with 20 nm fluorescent beads [left two columns in ( d , e )], which are in good agreement with the theoretical plot in ( b , c ). ( f ) The measured FOV profile of Gaussian light-sheet, LBS1 and LBS2. The Gaussian light-sheet is generated by taking away the annulus and confining the maximum NA to 0.5 so that the beam waist has a thickness of 600 nm, which is comparable to the thickness of LBS1. Scale bars in ( d , e ): 5 μm.

    Journal: Scientific Reports

    Article Title: Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets

    doi: 10.1038/srep26159

    Figure Lengend Snippet: ( a ) A simplified schematics illustrating LBS generation. L1: Collimating lens; CL1 and CL2: a pair of cylindrical lenses shaping the beam appropriately to maximize the energy passing through the single slit; L2: Fourier transform lens; EO: Excitation objective; DO: Detection objective. The beam is defined as propagating along y-axis. Using custom designed annuli one can craft LBS optimized for ( b ) ultralow phototoxicity (LBS1) or ( c ) high resolution (LBS2). In ( b , c ) from left to right: the annulus used and its numerical apertures (NA): NA max and NA min ; the diffraction pattern after the annulus; the cross-section of the LBS generated at sample plane; the intensity plot (blue) along the dotted yellow line and the axial point spread function of the NA = 1.1 detection objective (red); and the axial plot of overall point spread function. ( d , e ) show the experimentally generated LBSs at different wavelengths (488 nm for GFP and 560 nm for mCherry). The point spread functions were measured with 20 nm fluorescent beads [left two columns in ( d , e )], which are in good agreement with the theoretical plot in ( b , c ). ( f ) The measured FOV profile of Gaussian light-sheet, LBS1 and LBS2. The Gaussian light-sheet is generated by taking away the annulus and confining the maximum NA to 0.5 so that the beam waist has a thickness of 600 nm, which is comparable to the thickness of LBS1. Scale bars in ( d , e ): 5 μm.

    Article Snippet: A multiband emission filter designed for GFP and mCherry (Semrock) is placed in front of the tube lens to block unwanted illumination from the excitation laser.

    Techniques: CRAfT Assay, Generated